Proteome Interrogation Using Nanoprobes To Identify Targets of a Cancer-Killing Molecule

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• 作者：Liwen Li ; Qiu Zhang ; Aifeng Liu ; Xiue Li ; Hongyu Zhou ; Yin Liu ; Bing Yan
• 刊名：Journal of the American Chemical Society
• 出版年：2011
• 出版时间：May 11, 2011
• 年：2011
• 卷：133
• 期：18
• 页码：6886-6889
• 全文大小：775K
• 年卷期：v.133,no.18(May 11, 2011)
• ISSN：1520-5126

文摘

We report a generic approach for identification of target proteins of therapeutic molecules using nanoprobes. Nanoprobes verify the integrity of nanoparticle-bound ligands in live cells and pull down target proteins from the cellular proteome, providing very important information on drug targets and mechanisms of action. As an example, target proteins as 伪-tubulin and HSP 90 have been identified and validated.