Polymerized Planar Suspended Lipid Bilayers for Single Ion Channel Recordings: Comparison of Several Dienoyl Lipids

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• 作者：Benjamin A. Heitz ; Juhua Xu ; Ian W. Jones ; John P. Keogh ; Troy J. Comi ; Henry K. Hall ; Jr. ; Craig A. Aspinwall ; S. Scott Saavedra
• 刊名：Langmuir
• 出版年：2011
• 出版时间：March 1, 2011
• 年：2011
• 卷：27
• 期：5
• 页码：1882-1890
• 全文大小：900K
• 年卷期：v.27,no.5(March 1, 2011)
• ISSN：1520-5827

文摘

The stabilization of suspended planar lipid membranes, or black lipid membranes (BLMs), through polymerization of mono- and bis-functionalized dienoyl lipids was investigated. Electrical properties, including capacitance, conductance, and dielectric breakdown voltage, were determined for BLMs composed of mono-DenPC, bis-DenPC, mono-SorbPC, and bis-SorbPC both prior to and following photopolymerization, with diphytanoyl phosphocholine (DPhPC) serving as a control. Poly(lipid) BLMs exhibited significantly longer lifetimes and increased the stability of air鈭抴ater transfers. BLM stability followed the order bis-DenPC > mono-DenPC 鈮?mono-SorbPC > bis-SorbPC. The conductance of bis-SorbPC BLMs was significantly higher than that of the other lipids, which is attributed to a high density of hydrophilic pores, resulting in relatively unstable membranes. The use of poly(lipid) BLMs as matrices for supporting the activity of an ion channel protein (IC) was explored using 伪-hemolysin (伪-HL), a model IC. Characteristic i鈭?i>V plots of 伪-HL were maintained following photopolymerization of bis-DenPC, mono-DenPC, and mono-SorbPC, demonstrating the utility of these materials for preparing more durable BLMs for single-channel recordings of reconstituted ICs.