Rapid Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry Imaging with Scanning Desorption Laser Beam

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• 作者：Anton铆n Bedna艡铆k ; Pavel Kuba ; Eugene Moskovets ; Iva Tomalov谩 ; Pavel Kr谩sensk媒 ; Pavel Hou拧ka ; Jan Preisler
• 刊名：Analytical Chemistry
• 出版年：2014
• 出版时间：January 21, 2014
• 年：2014
• 卷：86
• 期：2
• 页码：982-986
• 全文大小：254K
• ISSN：1520-6882

文摘

Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI TOF MS) imaging of surfaces and tissues is a rapidly evolving technique having great potential in the field of biosciences. In earlier times, acquisition of a single high-resolution MS image could take days. Despite the recent introduction of high-repetition rate lasers to increase sample throughput of axial TOF MS instruments, obtaining a high-resolution image still requires a few hours. This paper shows that a substantial increase in the throughput of the TOF MS-based tissue imaging can be achieved by incorporating a mirror providing high-speed precision scanning of the laser beam along the sample surface. Equipped with the scanning mirror, a laboratory-built axial MALDI TOF MS instrument utilizing a 4-kHz UV laser recorded a 100 脳 100 pixel MS image in 11 min using 100 laser shots per pixel. This is almost an order of magnitude faster when compared to a modern commercial instrument equipped with 1-kHz laser.