Mass Spectrometric Proteomics Reveals that Nuclear Protein Positive Cofactor PC4 Selectively Binds to Cross-Linked DNA by a trans-Platinum Anticancer Complex

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• 作者：Zhifeng Du ; Qun Luo ; Liping Yang ; Tao Bing ; Xianchan Li ; Wei Guo ; Kui Wu ; Yao Zhao ; Shaoxiang Xiong ; Dihua Shangguan ; Fuyi Wang
• 刊名：Journal of the American Chemical Society
• 出版年：2014
• 出版时间：February 26, 2014
• 年：2014
• 卷：136
• 期：8
• 页码：2948-2951
• 全文大小：249K
• 年卷期：v.136,no.8(February 26, 2014)
• ISSN：1520-5126

文摘

An MS-based proteomic strategy combined with chemically functionalized gold nanoparticles as affinity probes was developed and validated by successful identification and quantification of HMGB1, which is well characterized to interact selectively with 1,2-cross-linked DNA by cisplatin, from whole cell lysates. The subsequent application of this method to identify proteins responding to 1,3-cross-linked DNA by a trans-platinum anticancer complex, trans-PtTz (Tz = thiazole), revealed that the human nuclear protein positive cofactor PC4 selectively binds to the damaged DNA, implying that PC4 may play a role in cellular response to DNA damage by trans-PtTz.