A Femtomolar Level and Highly Selective 17尾-estradiol Photoelectrochemical Aptasensor Applied in Environmental Water Samples Analysis

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• 作者：Lifang Fan ; Guohua Zhao ; Huijie Shi ; Meichuan Liu ; Yanbin Wang ; Hongyang Ke
• 刊名：Environmental Science & Technology
• 出版年：2014
• 出版时间：May 20, 2014
• 年：2014
• 卷：48
• 期：10
• 页码：5754-5761
• 全文大小：398K
• 年卷期：v.48,no.10(May 20, 2014)
• ISSN：1520-5851

文摘

Driven by the urgent demand of determining low level of 17尾-estradiol (E2) present in environment, a novel and ultrasensitive photoelectrochemical (PEC) sensing platform based on anti-E2 aptamer as the biorecognition element was developed onto CdSe nanoparticles-modified TiO₂ nanotube arrays. The designed PEC aptasensor exhibits excellent performances in determination of E2 with a wide linear range of 0.05鈥?5 pM. The detection limit of 33 fM is lower than the previous reports. The aptasensor manifests outstanding selectivity to E2 while used to detect seven other endocrine disrupting compounds that have similar structure or coexist with E2. The superior sensing behavior toward E2 can be attributed to the appropriate PEC sensing interface resulting from the preponderant tubular microstructure and excellent photoelectrical activity, the large packing density of aptamer on the sensing interface, as well as the high affinity of the aptamer to E2. The PEC aptasensor was applied successfully to determine E2 in environmental water samples without complicate sample pretreatments, and the analytical results showed good agreement with that determined by HPLC. Thus, a simple and rapid PEC technique for detection low level of E2 was established, having promising potential in monitoring environmental water pollution.