Allosteric Transition Induced by Mg2+ Ion in a Transactivator Monitored by SERS

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• 作者：Partha P. Kundu ; Tuhin Bhowmick ; Ganduri Swapna ; G. V. Pavan Kumar ; Valakunja Nagaraja ; Chandrabhas Narayana
• 刊名：Journal of Physical Chemistry B
• 出版年：2014
• 出版时间：May 22, 2014
• 年：2014
• 卷：118
• 期：20
• 页码：5322-5330
• 全文大小：458K
• 年卷期：v.118,no.20(May 22, 2014)
• ISSN：1520-5207

文摘

We demonstrate the utility of the surface-enhanced Raman spectroscopy (SERS) to monitor conformational transitions in protein upon ligand binding. The changes in protein鈥檚 secondary and tertiary structures were monitored using amide and aliphatic/aromatic side chain vibrations. Changes in these bands are suggestive of the stabilization of the secondary and tertiary structure of transcription activator protein C in the presence of Mg²⁺ ion, whereas the spectral fingerprint remained unaltered in the case of a mutant protein, defective in Mg²⁺ binding. The importance of the acidic residues in Mg²⁺ binding, which triggers an overall allosteric transition in the protein, is visualized in the molecular model. The present study thus opens up avenues toward the application of SERS as a potential tool for gaining structural insights into the changes occurring during conformational transitions in proteins.