Distance Determination in Proteins inside Xenopus laevis Oocytes by Double Electron−Electron Resonance Experiments

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• 作者：Ryuji Igarashi ; Tomomi Sakai ; Hideyuki Hara ; Takeshi Tenno ; Toshiaki Tanaka ; Hidehito Tochio ; Masahiro Shirakawa
• 刊名：Journal of the American Chemical Society
• 出版年：2010
• 出版时间：June 23, 2010
• 年：2010
• 卷：132
• 期：24
• 页码：8228-8229
• 全文大小：133K
• 年卷期：v.132,no.24(June 23, 2010)
• ISSN：1520-5126

文摘

DEER (double electron−electron resonance) enables the observation of long-range dipole interactions (1.5−8 nm) between electron spin centers and has become a unique method for structural analysis of site-directed spin-labeled (SDSL) proteins. The method was applied to proteins inside eukaryotic cells, Xenopus laevis oocytes. DEER measurements of the oocytes, into which SDSL-ubiquitin derivates were injected, gave rise to interpretable signals and allowed us to perform in situ analyses of the interspin distances of the proteins.