Comparison of 18F PET and 99mTc SPECT Imaging in Phantoms and in Tumored Mice

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• 作者：Dengfeng Cheng ; Yi Wang ; Xinrong Liu ; P. Hendrik Pretorius ; Minmin Liang ; Mary Rusckowski ; Donald J. Hnatowich
• 刊名：Bioconjugate Chemistry
• 出版年：2010
• 出版时间：August 18, 2010
• 年：2010
• 卷：21
• 期：8
• 页码：1565-1570
• 全文大小：304K
• 年卷期：v.21,no.8(August 18, 2010)
• ISSN：1520-4812

文摘

Our objective was to compare the performance of a micro-single photon emission computed tomography (micro-SPECT) with that of a micro-positron emission tomography (microPET) in a Her2+ tumored mice using an anti-Her2 nanoparticle radiolabeled with ^99mTc and ¹⁸F. Camera performance was first compared using phantoms; then a tumored mouse administered the ^99mTc-nanoparticle was imaged on a Bioscan NanoSPECT/CT, while another tumored mouse received the identical nanoparticle, labeled now with ¹⁸F, and was imaged on a Philips Mosaic HP PET camera. The nanoparticle was radiolabeled with ^99mTc via MAG₃ chelation and with ¹⁸F via SFB as an intermediate. Phantom imaging showed that the resolution of the SPECT camera was clearly superior, but even with 4 heads and multipinhole collimators, detection sensitivity was 15-fold lower. Radiolabeling of the nanoparticle by chelation with ^99mTc was considerably easier and safer than manual covalent attachment of ¹⁸F. Both cameras provided accurate quantitation of radioactivity over a broad range. In conclusion, when deciding between ^99mTc vs ¹⁸F, an advantage rests with the chelation of ^99mTc over covalent attachment of ¹⁸F, achieved manually or otherwise, but with these small animal cameras, this choice also results in trading lower sensitivity for higher resolution.