Malondialdehyde−Deoxyguanosine Adduct Formation in Workers of Pathology Wards: The Role of Air Formaldehyde Exposure

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• 作者：Roberto Bono ; Valeria Romanazzi ; Armelle Munnia ; Sara Piro ; Alessandra Allione ; Fulvio Ricceri ; Simonetta Guarrera ; Cristina Pignata ; Giuseppe Matullo ; Poguang Wang ; Roger W. Giese ; Marco Peluso
• 刊名：Chemical Research in Toxicology
• 出版年：2010
• 出版时间：August 16, 2010
• 年：2010
• 卷：23
• 期：8
• 页码：1342-1348
• 全文大小：219K
• 年卷期：v.23,no.8(August 16, 2010)
• ISSN：1520-5010

文摘

Formaldehyde is an ubiquitous pollutant to which humans are exposed. Pathologists can experience high formaldehyde exposure levels. Formaldehyde—among other properties—induce oxidative stress and free radicals, which react with DNA and lipids, leading to oxidative damage and lipid peroxidation, respectively. We measured the levels of air-formaldehyde exposure in a group of Italian pathologists and controls. We analyzed the effect of formaldehyde exposure on leukocyte malondialdehyde−deoxyguanosine adducts (M₁-dG), a biomarker of oxidative stress and lipid peroxidation. We studied the relationship between air-formaldehyde and M₁-dG adducts. Air-formaldehyde levels were measured by personal air samplers. M₁-dG adducts were analyzed by a ³²P-postlabeling assay. Reduction room pathologists were significantly exposed to air-formaldehyde with respect to controls and to the pathologists working in other laboratory areas (p < 0.001). A significant difference for M₁-dG adducts between exposed pathologists and controls was found (p = 0.045). The effect becomes stronger when the evaluation of air-formaldehyde exposure was based on personal samplers (p = 0.018). Increased M₁dG adduct levels were only found in individuals exposed to air-formaldehyde concentrations higher than 66 μg/m³. When the exposed workers and controls were subgrouped according to smoking, M₁-dG tended to increase in all of the subjects, but a significant association between M₁-dG and air-formaldehyde was only found in nonsmokers (p = 0.009). Air-formaldehyde played a role positive but not significant (r = 0.355, p = 0.075, Pearson correlation) in the formation of M₁-dG, only in nonsmokers. Working in the reduction rooms and exposure to air-formaldehyde concentrations higher than 66 μg/m³ are associated with increased levels of M₁-dG adducts.