Bacteriophage Bundles with Prealigned Ca2+ Initiate the Oriented Nucleation and Growth of Hydroxylapatite

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• 作者：Fuke Wang ; Binrui Cao ; Chuanbin Mao
• 刊名：Chemistry of Materials
• 出版年：2010
• 出版时间：June 22, 2010
• 年：2010
• 卷：22
• 期：12
• 页码：3630-3636
• 全文大小：842K
• 年卷期：v.22,no.12(June 22, 2010)
• ISSN：1520-5002

文摘

Inorganic ions may direct the self-assembly of biomacromolecules into nanostructures which can further be used as a reactant and matrix for nanomaterials synthesis and assembly. Here we use bone mineral and filamentous bacteriophage as a model to demonstrate this concept. Divalent calcium ions are found to trigger the electrostatic self-assembly of anionic nanofiber-like bacteriophages into bundle structures where calcium ions are preorganized between bacteriophage nanofibers. The resultant Ca²⁺-bacteriophage bundles can be separated and purified from the aqueous solution. The nanostructures of the bundles are verified by zeta potential analysis, small-angle X-ray scattering and transmission electron microscopy. Because of the transcription of the bacteriophage chiral surface to the periodic alignment of preloaded Ca²⁺, the Ca²⁺-bacteriphage bundles can serve as both Ca sources and biotemplates to initiate the oriented nucleation and growth of nanocrystalline hydroxyapatite in phosphate solution or in simulated body fluid. This work provides new insights into biomineralization and represents a new approach to the fabrication of biomolecular-inorganic hybrid layered nanostructures.