Effect of Molecular Weight and Degree of Substitution of Quaternized Cellulose on the Efficiency of Gene Transfection

详细信息    查看全文

• 作者：Yongbo Song ; Huiyuan Wang ; Xuan Zeng ; Yunxia Sun ; Xianzheng Zhang ; Jinping Zhou ; Lina Zhang
• 刊名：Bioconjugate Chemistry
• 出版年：2010
• 出版时间：July 21, 2010
• 年：2010
• 卷：21
• 期：7
• 页码：1271-1279
• 全文大小：449K
• 年卷期：v.21,no.7(July 21, 2010)
• ISSN：1520-4812

文摘

Quaternized celluloses (QCs) with different molecular weight (M_w) and degree of cationic substitution (DS) were homogeneously synthesized in NaOH/urea aqueous solutions and were studied as gene carriers. QCs were evaluated for efficacy of nanoparticle formation, DNA binding efficiency, morphology, and in vitro gene transfection efficiency. The factors affecting the transfection efficiency, e.g., M_w, DS, and N/P ratios, have been evaluated. The cytotoxicity of QCs and QC/DNA complexes were evaluated in 293T cells and were found to be relatively low compared with 25 kDa PEI and PEI/DNA complex, which increased slightly with increasing of M_w and DS. All QCs obtained could bind DNA efficiently, and QC/DNA complexes exhibited effective transfection in comparison to the naked DNA. More importantly, the QC/DNA complexes, were stable and the transfection efficiency was not inhibited in the presence of serum. The results revealed an important combined effect between M_w and DS of QCs in determining transgene expression, and QCs with M_w of about 8 × 10⁴ g/mol and DS of about 0.6 displayed relatively higher transfection efficiencies attributed to the intermediate stability.