Activity and Thermal Stability Improvements of Glucose Oxidase upon Adsorption on Core−Shell PMMA−BSA Nanoparticles

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• 作者：Chuanxin He ; Jianhong Liu ; Laiyong Xie ; Qianling Zhang ; Cuihua Li ; Dayong Gui ; Guangzhao Zhang ; Chi Wu
• 刊名：Langmuir
• 出版年：2009
• 出版时间：December 1, 2009
• 年：2009
• 卷：25
• 期：23
• 页码：13456-13460
• 全文大小：801K
• 年卷期：v.25,no.23(December 1, 2009)
• ISSN：1520-5827

文摘

The interaction and adsorption of enzyme, glucose oxidase (GOx), on poly(methyl methacrylate)−bovine serum albumin (PMMA−BSA) particles were studied by using a quartz crystal microbalance with dissipation (QCM-D) and laser light scattering (LLS). The enzyme was irreversibly immobilized on the PMMA−BSA particle surface. The amount of enzyme immobilized on PMMA−BSA particles and the enzymatic activity were determined by UV/vis measurements. The influences of pH and ionic strength on the adsorption indicate that the electrostatic interaction plays a major role on the immobilization. The adsorbed GOx can retain at least 80% of the free enzyme activity. Thermal stability studies reveal that the adsorbed GOx only losses 28% of its activity in comparison with a 64% activity loss of free GOx when it is incubated at 50 °C for 35 h.