Engineering of Streptomyces platensis MA7339 for Overproduction of Platencin and Congeners

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• 作者：Zhiguo Yu ; Michael J. Smanski ; Ryan M. Peterson ; Karen Marchillo ; David Andes ; Scott R. Rajski ; Ben Shen
• 刊名：Organic Letters
• 出版年：2010
• 出版时间：April 16, 2010
• 年：2010
• 卷：12
• 期：8
• 页码：1744-1747
• 全文大小：196K
• 年卷期：v.12,no.8(April 16, 2010)
• ISSN：1523-7052

文摘

Platensimycin (1) and platencin (2) are novel antibiotic leads against multidrug resistant pathogens. The production of 2 in Streptomyces platensis MA7339 is under the control of ptnR1, a GntR-like transcriptional regulator. Inactivating ptnR1 afforded S. platensis MA7339 mutant strain SB12600 that overproduces 2 at a titer 100-fold greater than that from the wild-type strain and accumulates platencin A₁ (3) and eight new congeners, platencins A₂−A₉ (4−11). The isolation, structural elucidation, and antibacterial activity of 4−11, in comparison to 1−3, are described.