Nanoparticles from Lipid-Based Liquid Crystals: Emulsifier Influence on Morphology and Cytotoxicity

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• 作者：Sergio Murgia ; Angela M. Falchi ; Miguel Mano ; Sandrina Lampis ; Rossella Angius ; Anna M. Carnerup ; Judith Schmidt ; Giacomo Diaz ; Mauro Giacca ; Yeshayahu Talmon ; Maura Monduzzi
• 刊名：Journal of Physical Chemistry B
• 出版年：2010
• 出版时间：March 18, 2010
• 年：2010
• 卷：114
• 期：10
• 页码：3518-3525
• 全文大小：312K
• 年卷期：v.114,no.10(March 18, 2010)
• ISSN：1520-5207

文摘

Here, monoolein-based nanoparticles (NPs), obtained through fragmentation of bulk liquid crystalline phases, and stabilized by two different emulsifiers, namely, Pluronic F127 (PF127) and lauroylcholine chloride (LCh), are investigated for structural features and for short-term in vitro cytotoxicity. Depending on the emulsifiers, different morphologies of the lipid NPs (cubosomes and liposomes) are obtained, as demonstrated by cryo-TEM images. Although NPs offer many advantages in medical applications and various chemicals used for their preparation are under investigation, so far there are no standardized procedures to evaluate cell biocompatibility. Two different protocols to evaluate the impact of these lipid NPs on biological systems are presented. Results show that nanoparticles stabilized by PF127 (cubosomes) display a relevant toxicity toward different cell lines, whereas those stabilized by LCh (liposomes) affect cell viability at a much lesser extent.