Unexpected Binding Modes of Nitric Oxide Synthase Inhibitors Effective in the Prevention of a Cerebral Palsy Phenotype in an Animal Model

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• 作者：Silvia L Delker ; Haitao Ji ; Huiying Li ; Joumana Jamal ; Jianguo Fang ; Fengtian Xue ; Richard B. Silverman ; Thomas L. Poulos
• 刊名：Journal of the American Chemical Society
• 出版年：2010
• 出版时间：April 21, 2010
• 年：2010
• 卷：132
• 期：15
• 页码：5437-5442
• 全文大小：239K
• 年卷期：v.132,no.15(April 21, 2010)
• ISSN：1520-5126

文摘

Selective inhibition of the neuronal isoform of nitric oxide synthase NOS (nNOS) has been shown to prevent brain injury and is important for the treatment of various neurodegenerative disorders. However, given the high active site conservation among all three NOS isoforms, the design of selective inhibitors is an extremely challenging problem. Here we present the structural basis for why novel and potent nNOS inhibitors exhibit the highest level of selectivity over eNOS reported so far (3,800-fold). By using a combination of crystallography, computational methods, and site-directed mutagenesis, we found that inhibitor chirality and an unanticipated structural change of the target enzyme control both the orientation and selectivity of these novel nNOS inhibitors. A new hot spot generated as a result of enzyme elasticity provides important information for the future fragment-based design of selective NOS inhibitors.