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Composition of Transgenic Volvariella volvacea Tolerant to Cold Stress Is Equivalent to That of Conventional Control
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  • 作者:Jie Wang ; ; ; Liqiong Guo ; ; Junfang Lin* ; ;
  • 刊名:Journal of Agricultural and Food Chemistry
  • 出版年:2009
  • 出版时间:March 25, 2009
  • 年:2009
  • 卷:57
  • 期:6
  • 页码:2392-2396
  • 全文大小:103K
  • 年卷期:v.57,no.6(March 25, 2009)
  • ISSN:1520-5118
文摘
Transgenic Volvariella volvacea strains, which are tolerant to cold stress, were generated by the stable insertion of antifreeze protein gene isolated from budworm into the genome of a conventional variety V23 of V. volvacea. As a part of the safety assessment program, transgenic V. volvacea strains were compared to a nontransgenic near-isogenic V. volvacea strain V23 grown contemporaneously by applying the principle of substantial equivalence. Compositional analyses were conducted by measuring proximates, amino acids, fatty acids, minerals, vitamins, 5′-nucleotides, nucleic acid, and antinutrients such as tannin and cyanide in V. volvacea strains harvested at egg-shaped stage. Results of the comparisons indicate that these transgenic strains are compositionally equivalent to the conventional control.

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