文摘
Transgenic Volvariella volvacea strains, which are tolerant to cold stress, were generated by the stable insertion of antifreeze protein gene isolated from budworm into the genome of a conventional variety V23 of V. volvacea. As a part of the safety assessment program, transgenic V. volvacea strains were compared to a nontransgenic near-isogenic V. volvacea strain V23 grown contemporaneously by applying the principle of substantial equivalence. Compositional analyses were conducted by measuring proximates, amino acids, fatty acids, minerals, vitamins, 5′-nucleotides, nucleic acid, and antinutrients such as tannin and cyanide in V. volvacea strains harvested at egg-shaped stage. Results of the comparisons indicate that these transgenic strains are compositionally equivalent to the conventional control.