Diversity-Oriented Fluorescence Library Approach (DOFLA) to the Discovery of Chymotrypsin Sensor

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• 作者：Shenliang Wang ; Yun Kyung Kim ; Young-Tae Chang
• 刊名：Journal of Combinatorial Chemistry
• 出版年：2008
• 出版时间：May 2008
• 年：2008
• 卷：10
• 期：3
• 页码：460 - 465
• 全文大小：604K
• 年卷期：v.10,no.3(May 2008)
• ISSN：1520-4774

文摘

The diversity-oriented fluorescence library approach (DOFLA) has emerged and found applications in various fields to meet the acute demands for novel fluorescence sensors. The power of this approach has been demonstrated with the impressive discoveries of novel sensors for polymers such as DNA and heparin or for small molecules such as GTP and glutathione (J. Am. Chem. Soc. 2003, 125, 1130− 1131; J. Am. Chem. Soc. 2006, 128, 10380− 10381; J. Am. Chem. Soc. 2007, 129, 4510− 4511; Chem. Commun. [Online early access]. DOI: 10.1039/b717058k. Published online Dec 11, 2008. http://www.rsc.org/publishing/journals/CC/article.asp?doi=b717058k). Herein we report the application of this approach on quinaldinium fluorescent dye library synthesis on solid support and novel chymotrypsin sensor discovery. The new sensors are not only selective to chymotrypsin over other proteins but also only to the active conformation of chymotrypsin.