Sulfated β-Glucan Derived from Oat Bran with Potent Anti-HIV Activity

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• 作者：Shun Chun Wang ; S. W. Annie Bligh ; Chang Lian Zhu ; Song Shan Shi ; Zheng Tao Wang ; Zhi Bi Hu ; John Crowder ; Christopher Branford-White ; Cherelyn Vella
• 刊名：Journal of Agricultural and Food Chemistry
• 出版年：2008
• 出版时间：April 23, 2008
• 年：2008
• 卷：56
• 期：8
• 页码：2624 - 2629
• 全文大小：389K
• 年卷期：v.56,no.8(April 23, 2008)
• ISSN：1520-5118

文摘

China is a major producer of oats; the annual harvested area of 350,000 ha yields approximately 465,000 tons, giving an average yield of 1.33 tons/ha. The bran is not used for animal feed as it is of poor digestibility and low nutritive content and is considered a waste byproduct. Therefore, it is advantageous to produce a value-added product from the bran. We extracted the native polysaccharide, a linear (1–3)-, (1–4)-linked β-glucan (OBG) from the oat bran and synthesized a sulfated derivative OBGS containing 36.5% sulfate. OBGS had potent activity against a primary isolate of human immunodeficiency virus (HIV-1) in peripheral blood mononuclear cells at a concentration (EC₅₀ = 5.98 ×10⁻⁴ µM) approximately 15,000 times below its cytotoxic concentration. OBGS was also active postinfection (EC₅₀ = 5.3 ×10⁻⁴ µM) and protected pretreated peripheral mononuclear cells (EC₅₀ = 5.2 ×10⁻² µM) washed free of the compounds prior to infection. Thus, OBGS has potential as a vaginal microbicide and is the first such report for oat bran derived sulfated̃ β-glucan.