Enhancement of Mussel Adhesive Protein Production in Escherichia coli by Co-expression of Bacterial Hemoglobin

详细信息    查看全文

• 作者：Doil Kim ; Dong Soo Hwang ; Dong Gyun Kang ; Jaoon Y. H. Kim ; Hyung Joon Cha
• 刊名：Biotechnology Progress
• 出版年：2008
• 出版时间：June 2008
• 年：2008
• 卷：24
• 期：3
• 页码：663 - 666
• 全文大小：237K
• 年卷期：v.24,no.3(June 2008)
• ISSN：1520-6033

文摘

Mussel adhesive proteins (MAPs) have been considered as potential underwater and medicalbioadhesives. Previously, we reported a functional expression of recombinant MAP hybrid fp-151, which is a fusion protein with six type 1 (fp-1) decapeptide repeats at each type 5 (fp-5)terminus, with practical properties in Escherichia coli. In the present work, we introduced theVitreoscilla hemoglobin (VHb) co-expression strategy to enhance the production levels of hybridfp-151 since VHb has been successfully used for efficient oxygen utilization in several expressionsystems, including E. coli. In both batch-type flask and fed-batch-type bioreactor cultures, wefound that co-expression of VHb conferred higher cell growth and hybrid fp-151 production. Itspositive effects were significantly increased in high cell density bioreactor cultures as themicroaerobic environment was more quickly and severely formed. We obtained a ~1.9-foldhigher (~1 g/L) production of MAP fp-151 from VHb co-expressing cells in fed-batch bioreactorcultures as compared to that from VHb non-expressing cells. Collectively and regardless of theculture type, VHb co-expression strategy was successful in enhancing the production ofrecombinant mussel adhesive proteins in the E. coli expression system.