Is the Oxidation of High-Density Lipoprotein Lipids Different Than the Oxidation of Low-Density Lipoprotein Lipids?

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• 作者：Michael J. Thomas ; Quiri Chen ; Manal Zabalawi ; Rachel Anderson ; Martha Wilson ; Richard Weinberg ; Mary G. Sorci-Thomas ; and Lawrence L. Rudel
• 刊名：Biochemistry
• 出版年：2001
• 出版时间：February 13, 2001
• 年：2001
• 卷：40
• 期：6
• 页码：1719 - 1724
• 全文大小：61K
• 年卷期：v.40,no.6(February 13, 2001)
• ISSN：1520-4995

文摘

This article gives detailed insight into the kinetics of high-density lipoprotein (HDL) oxidationcatalyzed by azobis(2-amidinopropane)·dihydrochloride (ABAP) or by copper. ABAP initialized oxidationof human HDL 3-4 times faster than non-human primate HDL with a similar composition. The oxidizabilityof non-human primate HDL was 1000 times lower than the oxidizability calculated from rate constantsderived from liposome oxidation, suggesting that there is a slow step in HDL oxidation not present inliposomes. Saturable binding of copper to HDL was a significant feature of copper-catalyzed oxidation.Binding constants (K_m) for non-human primate HDL were 2-3-fold lower than those for human HDL.Copper-catalyzed oxidation of non-human primate HDL was slower than that of human HDL, but humanHDL₂ and HDL₃ oxidized at about the same rate. Overall, the kinetics describing the oxidation of HDLwere mechanistically similar to those reported for LDL, suggesting that HDL lipids were as easily oxidizedas LDL lipids and that HDL will be easily oxidized in vivo when exposed to agents that oxidize LDL.