Sex Dependence of the Components and Structure of Urinary Calculi Induced by Biphenyl Administration in Rats

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• 作者：Makoto Ohnishi ; Hirofumi Yajima ; Seigo Yamamoto ; Taijiro Matsushima ; and Tadahiro Ishii
• 刊名：Chemical Research in Toxicology
• 出版年：2000
• 出版时间：August 2000
• 年：2000
• 卷：13
• 期：8
• 页码：727 - 735
• 全文大小：2902K
• 年卷期：v.13,no.8(August 2000)
• ISSN：1520-5010

文摘

To obtain definitive information about the mechanisms of urinary calculus formation andthe structural characteristics of the calculi induced by biphenyl administration in rats, with afocus on the sex dependency, the constituents of the urinary calculi were analyzed by HPLC,inductively coupled plasma spectroscopy (ICP), micro Fourier transform infrared spectroscopy(mFT-IR), and ion chromatography (IC), and structural analyses were carried out by microscopy,mFT-IR, and the electron probe microanalyzer (EPMA) method. We attempted to account forthe appreciably higher incidence of calculi in males than in females. mFT-IR analysis revealedthat the biphenyl-induced urinary calculi in male rats are composed mainly of potassium4-hydroxybiphenyl-o-sulfate (4-HBPOSK), whereas the calculi in female rats are composedmainly of 4-hydroxybiphenyl (4-HBP) and KHSO₄ produced by the hydrolysis of 4-HBPOSK.Observations of photomicrographs and the results of mFT-IR analysis indicated that the calculiin males have a multilayer structure consisting of alternating layers of 4-HBPOSK and calciumphosphate, whereas the calculi in females have no multilayer structure, but open holes inwhich needle-shaped crystals are present in some places. In view of the results of these analyses,including the EPMA analysis, it appears that calculus formation in males may involve a seriesof successive and irreversible reactions, whereas calculus formation in females may result froma series of reversible reactions, including the hydrolysis of 4-HBPOSK. It was inferred thatthe series of irreversible reactions involved in calculus formation in males is relatively morestable than that in the case of females, and thus, a sex difference in the reaction features maybe responsible for the observed difference in the incidence of calculus formation.