Label-Free Catalytic and Molecular Beacon Containing an Abasic Site for Sensitive Fluorescent Detection of Small Inorganic and Organic Molecules

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• 作者：Panshu Song ; Yu Xiang ; Hang Xing ; Zhaojuan Zhou ; Aijun Tong ; Yi Lu
• 刊名：Analytical Chemistry
• 出版年：2012
• 出版时间：March 20, 2012
• 年：2012
• 卷：84
• 期：6
• 页码：2916-2922
• 全文大小：305K
• 年卷期：v.84,no.6(March 20, 2012)
• ISSN：1520-6882

文摘

In this work, two methods with complementary features, catalytic and molecular beacon (CAMB) and label-free fluorescent sensors using an abasic site, have been combined into new label-free CAMB sensors that possess advantages of each method. The label-free method using a dSpacer-containing molecular beacon makes CAMB more cost-effective and less interfering with the catalytic activity, while CAMB allows the label-free method to use true catalytic turnovers for signal amplifications, resulting in a new label-free CAMB sensor for Pb²⁺ ion, with a detection limit of 3.8 nM while maintaining the same selectivity. Furthermore, by using CAMB to overcome the label-free method鈥檚 limitation of requiring excess enzyme strands, a new label-free CAMB sensor using aptazyme is also designed to detect adenosine down to 1.4 渭M, with excellent selectivity over other nucleosides.