Stimulated Release of Cholesterol from Liposomal Membranes by a PEGylated Phospholipid

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• 作者：Vaclav Janout ; Trevor A. Daly ; Lauren L. Cline ; Leah J. Kulp ; Steven L. Regen
• 刊名：Bioconjugate Chemistry
• 出版年：2012
• 出版时间：March 21, 2012
• 年：2012
• 卷：23
• 期：3
• 页码：336-339
• 全文大小：220K
• 年卷期：v.23,no.3(March 21, 2012)
• ISSN：1520-4812

文摘

PEGylated phospholipids are commonly used to increase the blood-circulation time of liposomes by providing a steric barrier around them. This paper documents a fundamentally new property of these lipids鈥攁n ability to stimulate the release of cholesterol from phospholipid membranes. Evidence for such stimulation has been obtained by measuring the transport of dehydroergosterol (DHE), a fluorescent simulant of cholesterol, from donor liposomes made from 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC), 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[methoxy(polyethylene glycol)-2000 (DSPE-PEG₂₀₀₀), and DHE to acceptor liposomes made from POPC, 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphoglycerol (POPG), and cholesterol. The potential of PEGylated lipids to serve as novel cholesterol-lowering agents is briefly discussed.