Ingestion of Proteoglycan Fraction from Shark Cartilage Increases Serum Inhibitory Activity against Matrix Metalloproteinase-9 and Suppresses Development of N-Nitrosobis(2-oxopropyl)amine-Induc

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• 作者：Tsukasa Kitahashi ; Shoko Ikawa ; Akika Sakamoto ; Yoshihiro Nomura ; Toshifumi Tsujiuchi ; Kenji Shimizu ; Shuji Sasabe ; Eun Young Park ; Yasushi Nakamura ; Masahiro Tsutsumi ; Kenji Sato
• 刊名：Journal of Agricultural and Food Chemistry
• 出版年：2012
• 出版时间：February 1, 2012
• 年：2012
• 卷：60
• 期：4
• 页码：940-945
• 全文大小：224K
• 年卷期：v.60,no.4(February 1, 2012)
• ISSN：1520-5118

文摘

A water extract of shark cartilage was fractionated into acidic and basic fractions by preparative isoelectric focusing on the basis of the amphoteric nature of samples. The acidic fraction was further fractionated into ethanol-soluble and -precipitate fractions. After the carcinogenesis treatment using N-nitrosobis(2-oxopropyl)amine, hamsters received a diet containing each fraction or purified chondroichin sulfate to give 0.4% (w/w) for 50 days. Only administration of the acidic ethanol-precipitate-fraction-containing diet significantly increased serum inhibitory activity against matrix metalloproteinase (MMP)-9 and reduced the number of adenocarcinomas in the pancreatic duct. The active fraction predominantly consisted of chondroichin sulfate-containing proteoglycan. However, the purified chondroichin sulfate had no significant activity. These results suggest that the protein moiety of the proteoglycan might be involved in the increase of serum inhibitory activity against MMP-9 and suppression of pancreatic carcinogenesis in hamster.

Keywords:

cancer; matrix metalloproteinase; MMP; MMP-9; shark cartilage; shark; cartilage; BOP; N-nitrosobis(2-oxopropyl)amine; carcinogenesis; proteoglycan; chondroichin sulfate; hamster