Licochalcone A Inhibits Lipopolysaccharide-Induced Inflammatory Response in Vitro and in Vivo

详细信息    查看全文

• 作者：Xiao Chu ; Xinxin Ci ; Miaomiao Wei ; Xiaofeng Yang ; Qingjun Cao ; Mingfeng Guan ; Hongyu Li ; Yanhong Deng ; Haihua Feng ; Xuming Deng
• 刊名：Journal of Agricultural and Food Chemistry
• 出版年：2012
• 出版时间：April 18, 2012
• 年：2012
• 卷：60
• 期：15
• 页码：3947-3954
• 全文大小：446K
• 年卷期：v.60,no.15(April 18, 2012)
• ISSN：1520-5118

文摘

Licochalcone A (Lico A), a flavonoid found in licorice root (Glycyrrhiza glabra), is known for its antimicrobial activity and its reported ability to inhibit cancer cell proliferation. In the present study, we found that Lico A exerted potent anti-inflammatory effects in in vitro and in vivo models induced by lipopolysaccharide (LPS). The concentrations of TNF-伪, interleukin (IL)-6, and IL-1尾 in the culture supernatants of RAW 264.7 cells were determined at different time points following LPS administration. LPS (0.5 mg/kg) was instilled intranasally (i.n.) in phosphate-buffered saline to induce acute lung injury, and 24 h after LPS was given, bronchoalveolar lavage fluid was obtained to measure pro-inflammatory mediator and total cell counts. The phosphorylation of mitogen-activated protein kinases (MAPKs) and nuclear factor-魏B (NF-魏B) p65 protein was analyzed by Western blotting. Our results showed that Lico A significantly reduced the amount of inflammatory cells, the lung wet-to-dry weight (W/D) ratio, protein leakage, and myeloperoxidase activity and enhances oxidase dimutase activity in mice with LPS-induced acute lung injury (ALI). Enzyme-linked immunosorbent assay results indicated that Lico A can significantly down-regulate TNF-伪, IL-6, and IL-1尾 levels in vitro and in vivo, and treatment with Lico A significantly attenuated alveolar wall thickening, alveolar hemorrhage, interstitial edema, and inflammatory cells infiltration in mice with ALI. In addition, we further demonstrated that Lico A exerts an anti-inflammation effect in an in vivo model of acute lung injury through suppression of NF-魏B activation and p38/ERK MAPK signaling in a dose-dependent manner.

Keywords:

licochalcone A; lipopolysaccharide; nuclear factor-魏B; mitogen-activated protein kinase