Enantioselective Analysis of Triazole Fungicide Myclobutanil in Cucumber and Soil under Different Application Modes by Chiral Liquid Chromatography/Tandem Mass Spectrometry

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• 作者：Fengshou Dong ; Li Cheng ; Xingang Liu ; Jun Xu ; Jing Li ; Yuanbo Li ; Zhiqiang Kong ; Qiu Jian ; Yongquan Zheng
• 刊名：Journal of Agricultural and Food Chemistry
• 出版年：2012
• 出版时间：February 29, 2012
• 年：2012
• 卷：60
• 期：8
• 页码：1929-1936
• 全文大小：347K
• 年卷期：v.60,no.8(February 29, 2012)
• ISSN：1520-5118

文摘

A sensitive and enantioselective method was developed and validated for the determination of myclobutanil enantiomers by chiral liquid chromatography coupled with tandem mass spectrometry. The separation and determination were performed using reversed-phase chromatography on a Chiralcel OD-RH column, with ACN鈥搘ater (70/30, v/v) as the mobile phase under isocratic conditions at 0.5 mL/min flow rate. The matrix effect, linearity, precision, accuracy, and stability were evaluated. The proposed method then was successfully applied to the study of enantioselective degradation of rac-myclobutanil in cucumber and soil under different application modes. The results showed that the preferential degradation of (+)-myclobutanil resulted in an enrichment of the (鈭?-myclobutanil residue in plant and soil. Moreover, in cucumber, the stereoselective intensity of myclobutanil under root douche treatment was stronger than that under foliar spraying treatment, whereas in soil, the intensity was exactly opposite. The probable reasons underlying these enantioselective effects were also discussed. This study highlighted the importance of examining the fate of both enantiomers in the greenhouse system for the correct use of chiral pesticides.

Keywords:

myclobutanil; enantioselective; application modes; cucumber; soil