1,3,8-Triazaspiro[4.5]decane-2,4-diones as Efficacious Pan-Inhibitors of Hypoxia-Inducible Factor Prolyl Hydroxylase 1鈥? (HIF PHD1鈥?) for the Treatment of Anemia

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• 作者：Petr Vachal ; Shouwu Miao ; Joan M. Pierce ; Deodial Guiadeen ; Vincent J. Colandrea ; Matthew J. Wyvratt ; Scott P. Salowe ; Lisa M. Sonatore ; James A. Milligan ; Richard Hajdu ; Anantha Gollapudi ; Carol A. Keohane ; Russell B. Lingham ; Suzanne M. Mandala ; Julie A. DeMartino ; Xinchun Tong ; Michael Wolff ; Dietrich Steinhuebel ; Gerard R. Kieczykowski ; Fred J. Fleitz ; Kevin Chapman ; John Athanasopoulos ; Gregory Adam ; Can D. Akyuz ; Dhirendra K. Jena ; Jeffrey W. Lusen ; Juncai Meng ; Benjamin D. Stein ; Lei Xia ; Edward C. Sherer ; Jeffrey J. Hale
• 刊名：Journal of Medicinal Chemistry
• 出版年：2012
• 出版时间：April 12, 2012
• 年：2012
• 卷：55
• 期：7
• 页码：2945-2959
• 全文大小：723K
• 年卷期：v.55,no.7(April 12, 2012)
• ISSN：1520-4804

文摘

The discovery of 1,3,8-triazaspiro[4.5]decane-2,4-diones (spirohydantoins) as a structural class of pan-inhibitors of the prolyl hydroxylase (PHD) family of enzymes for the treatment of anemia is described. The initial hit class, spirooxindoles, was identified through affinity selection mass spectrometry (AS-MS) and optimized for PHD2 inhibition and optimal PK/PD profile (short-acting PHDi inhibitors). 1,3,8-Triazaspiro[4.5]decane-2,4-diones (spirohydantoins) were optimized as an advanced lead class derived from the original spiroindole hit. A new set of general conditions for C鈥揘 coupling, developed using a high-throughput experimentation (HTE) technique, enabled a full SAR analysis of the spirohydantoins. This rapid and directed SAR exploration has resulted in the first reported examples of hydantoin derivatives with good PK in preclinical species. Potassium channel off-target activity (hERG) was successfully eliminated through the systematic introduction of acidic functionality to the molecular structure. Undesired upregulation of alanine aminotransferese (ALT) liver enzymes was mitigated and a robust on-/off-target margin was achieved. Spirohydantoins represent a class of highly efficacious, short-acting PHD1鈥? inhibitors causing a robust erythropoietin (EPO) upregulation in vivo in multiple preclinical species. This profile deems spirohydantoins as attractive short-acting PHDi inhibitors with the potential for treatment of anemia.