Metabonomic Profiles Discriminate Hepatocellular Carcinoma from Liver Cirrhosis by Ultraperformance Liquid Chromatography鈥揗ass Spectrometry

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• 作者：Baohong Wang ; Deying Chen ; Yu Chen ; Zhenhua Hu ; Min Cao ; Qing Xie ; Yanfei Chen ; Jiali Xu ; Shusen Zheng ; Lanjuan Li
• 刊名：Journal of Proteome Research
• 出版年：2012
• 出版时间：February 3, 2012
• 年：2012
• 卷：11
• 期：2
• 页码：1217-1227
• 全文大小：457K
• 年卷期：v.11,no.2(February 3, 2012)
• ISSN：1535-3907

文摘

Hepatocellular carcinoma (HCC) is the fifth most common cancer worldwide and usually develops in patients with liver cirrhosis (LC). Biomarkers that discriminate HCC from LC are important but are limited. In the present study, an ultraperformance liquid chromatography鈥搈ass spectrometry (UPLC鈥揗S)-based metabonomics approach was used to characterize serum profiles from HCC (n = 82), LC (n = 48), and healthy subjects (n = 90), and the accuracy of UPLC鈥揗S profiles and alpha-fetoprotein (AFP) levels were compared for their use in HCC diagnosis. By multivariate data and receiver operating characteristic curves analysis, metabolic profiles were capable of discriminating not only patients from the controls but also HCC from LC with 100% sensitivity and specificity. Thirteen potential biomarkers were identified and suggested that there were significant disturbances of key metabolic pathways, such as organic acids, phospholipids, fatty acids, bile acids, and gut flora metabolism, in HCC patients. Canavaninosuccinate was first identified as a metabolite that exhibited a significant decrease in LC and an increase in HCC. In addition, glycochenodeoxycholic acid was suggested to be an important indicator for HCC diagnosis and disease prognosis. UPLC鈥揗S signatures, alone or in combination with AFP levels, could be an efficient and convenient tool for early diagnosis and screening of HCC in high-risk populations.

Keywords:

serum; metabonomics; hepatocellular carcinoma (HCC); liver cirrhosis (LC); liquid chromatography鈭抦ass spectrometry (LC鈭扢S)