Delivery of Multiple siRNAs Using Lipid-Coated PLGA Nanoparticles for Treatment of Prostate Cancer

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• 作者：Warefta Hasan ; Kevin Chu ; Anuradha Gullapalli ; Stuart S. Dunn ; Elizabeth M. Enlow ; J. Christopher Luft ; Shaomin Tian ; Mary E. Napier ; Patrick D. Pohlhaus ; Jason P. Rolland ; Joseph M. DeSimone
• 刊名：Nano Letters
• 出版年：2012
• 出版时间：January 11, 2012
• 年：2012
• 卷：12
• 期：1
• 页码：287-292
• 全文大小：355K
• 年卷期：v.12,no.1(January 11, 2012)
• ISSN：1530-6992

文摘

Nanotechnology can provide a critical advantage in developing strategies for cancer management and treatment by helping to improve the safety and efficacy of novel therapeutic delivery vehicles. This paper reports the fabrication of poly(lactic acid-co-glycolic acid)/siRNA nanoparticles coated with lipids for use as prostate cancer therapeutics made via a unique soft lithography particle molding process called Particle Replication In Nonwetting Templates (PRINT). The PRINT process enables high encapsulation efficiency of siRNA into neutral and monodisperse PLGA particles (32鈥?6% encapsulation efficiency). Lipid-coated PLGA/siRNA PRINT particles were used to deliver therapeutic siRNA in vitro to knockdown genes relevant to prostate cancer.

Keywords:

Soft lithography; siRNA delivery; PLGA; lipids; PRINT; prostate cancer