A Turn-on Fluorescent Sensor for Pyrophosphate Based on the Disassembly of Cu2+-Mediated Perylene Diimide Aggregates

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• 作者：Xueling Feng ; Yuxiu An ; Zhiyi Yao ; Chun Li ; Gaoquan Shi
• 刊名：ACS Applied Materials & Interfaces
• 出版年：2012
• 出版时间：February 22, 2012
• 年：2012
• 卷：4
• 期：2
• 页码：614-618
• 全文大小：304K
• 年卷期：v.4,no.2(February 22, 2012)
• ISSN：1944-8252

文摘

A complex between an anionic perylene diimide derivative (PDI-GlyAsp) and cupric ion has been prepared and applied to be turn-on fluorescent probe for the detection of pyrophosphate (PPi) in 100% aqueous solution. The complex formation process and PPi detection have been studied by absorption and emission spectroscopy. It was confirmed that the introduction of cupric ion into PDI-GlyAsp solution resulted in the assembly of PDI-GlyAsp into PDI-GlyAsp/Cu²⁺ aggregates, leading to the fluorescence quenching of PDI-GlyAsp. Upon addition of PPi into the above solution led to the disassembly of the aggregates due to the competitive binding of PPi with Cu²⁺ in the PDI-GlyAsp/Cu²⁺ complex, and a recovery of PDI-GlyAsp emission was observed. Therefore, the PDI-GlyAsp/Cu²⁺ complex can be applied as a turn-on fluorescent probe for detecting PPi with high selectivity and sensitivity.

Keywords:

pyrophosphate; perylene diimide; fluorescence; biosensor; aggregation