Resolving Low-Expression Cell Surface Antigens by Time-Gated Orthogonal Scanning Automated Microscopy

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• 作者：Jie Lu ; Jody Martin ; Yiqing Lu ; Jiangbo Zhao ; Jingli Yuan ; Martin Ostrowski ; Ian Paulsen ; James A. Piper ; Dayong Jin
• 刊名：Analytical Chemistry
• 出版年：2012
• 出版时间：November 20, 2012
• 年：2012
• 卷：84
• 期：22
• 页码：9674-9678
• 全文大小：314K
• 年卷期：v.84,no.22(November 20, 2012)
• ISSN：1520-6882

文摘

We report a highly sensitive method for rapid identification and quantification of rare-event cells carrying low-abundance surface biomarkers. The method applies lanthanide bioprobes and time-gated detection to effectively eliminate both nontarget organisms and background noise and utilizes the europium containing nanoparticles to further amplify the signal strength by a factor of 20. Of interest is that these nanoparticles did not correspondingly enhance the intensity of nonspecific binding. Thus, the dramatically improved signal-to-background ratio enables the low-expression surface antigens on single cells to be quantified. Furthermore, we applied an orthogonal scanning automated microscopy (OSAM) technique to rapidly process a large population of target-only cells on microscopy slides, leading to quantitative statistical data with high certainty. Thus, the techniques together resolved nearly all false-negative events from the interfering crowd including many false-positive events.