Silicon Nanowire-Based Molecular Beacons for High-Sensitivity and Sequence-Specific DNA Multiplexed Analysis

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• 作者：Shao Su ; Xinpan Wei ; Yiling Zhong ; Yuanyuan Guo ; Yuanyuan Su ; Qing Huang ; Shuit-Tong Lee ; Chunhai Fan ; Yao He
• 刊名：ACS Nano
• 出版年：2012
• 出版时间：March 27, 2012
• 年：2012
• 卷：6
• 期：3
• 页码：2582-2590
• 全文大小：1048K
• 年卷期：v.6,no.3(March 27, 2012)
• ISSN：1936-086X

文摘

Nanomaterial-based molecular beacons (nanoMBs) have been extensively explored due to unique merits of nanostructures, including gold nanoparticle (AuNP)-, carbon nanotube (CNT)-, and graphene-based nanoMBs. Those nanoMBs are well-studied; however, they possess relatively poor salt stability or low specificity, limiting their wide applications. Here, we present a novel kind of multicolor silicon-based nanoMBs by using AuNP-decorated silicon nanowires as high-performance quenchers. Significantly, the nanoMBs feature robust stability in high-concentration (0.1 M) salt solution and wide-ranging temperature (10鈥?0 掳C), high quenching efficiency (>90%) for various fluorophores (e.g., FAM, Cy5, and ROX), and large surfaces for simultaneous assembly of different DNA strands. We further show that silicon-based nanoMBs are highly effective for sensitive and specific multidetection of DNA targets. The unprecedented advantages of silicon-based multicolor nanoMBs would bring new opportunities for challenging bioapplications, such as allele discrimination, early cancer diagnosis, and molecular engineering, etc.

Keywords:

molecular beacons; silicon nanowires; gold nanoparticles; DNA; multianalysis