Accumulation and Biotransformation of BDE-47 by Zebrafish Larvae and Teratogenicity and Expression of Genes along the Hypothalamus鈥揚ituitary鈥揟hyroid Axis

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• 作者：Xinmei Zheng ; Yuting Zhu ; Chunsheng Liu ; Hongling Liu ; John P. Giesy ; Markus Hecker ; Michael H. W. Lam ; Hongxia Yu
• 刊名：Environmental Science & Technology (ES&T)
• 出版年：2012
• 出版时间：December 4, 2012
• 年：2012
• 卷：46
• 期：23
• 页码：12943-12951
• 全文大小：408K
• 年卷期：v.46,no.23(December 4, 2012)
• ISSN：1520-5851

文摘

Accumulation and effects of BDE-47 and two analogues, 6-OH-BDE-47 and 6-MeO-BDE-47, on ontogeny and profiles of transcription of genes along the hypothalamus鈥損ituitary鈥搕hyroid (HPT) axis of zebrafish (Danio rerio) embryos exposed from 4 h post fertilization (hpf) to 120 hpf were investigated. The 96 h-LC₅₀ of the most toxic compound, based on teratogenicity, was 330 渭g of 6-OH-BDE-47/L. 6-OH-BDE-47 significantly down-regulated expression of mRNA of thyroid stimulating hormone receptor (TSHR), thyroid hormone receptors (TRs, including TR伪 and TR尾), sodium/iodide symporter (NIS), and transthyretin (TTR) while up-regulating expression of thyroglobulin (TG) and thyrotropin-releasing hormone (TRH). Spontaneous movement was affected by 1 mg of 6-OH-BDE-47/L or 5 mg of 6-MeO-BDE-47/L. BDE-47 did not alter activity of larvae at any concentration tested. 6-MeO-BDE-47 significantly up-regulated expression of mRNA of TRH, TR伪, TR尾 and NIS. Both 6-OH-BDE-47 and 6-MeO-BDE-47 affected the thyroid hormone pathway. BDE-47 and 6-MeO-BDE-47 were accumulated more than 6-OH-BDE-47. 6-MeO-BDE-47 was transformed into 6-OH-BDE-47, but BDE-47 was not transformed into it. In summary, the synthetic brominated flame retardant, BDE-47, did not elicit the adverse effects caused by the other two analogues and appeared to have less toxicological relevance than the two natural product analogues 6-OH- and 6-MeO-BDE-47.