In situ Electrochemical-Transmission Surface Plasmon Resonance Spectroscopy for Poly(pyrrole-3-carboxylic acid) Thin-Film-Based Biosensor Applications

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• 作者：Rapiphun Janmanee ; Akira Baba ; Sukon Phanichphant ; Saengrawee Sriwichai ; Kazunari Shinbo ; Keizo Kato ; Futao Kaneko
• 刊名：ACS Applied Materials & Interfaces
• 出版年：2012
• 出版时间：August 22, 2012
• 年：2012
• 卷：4
• 期：8
• 页码：4270-4275
• 全文大小：336K
• 年卷期：v.4,no.8(August 22, 2012)
• ISSN：1944-8252

文摘

In this study, we describe the combination of transmission surface plasmon resonance (TSPR) and electrochemical techniques for the application to biosensors with conducting polymers. Electropolymerization was employed to construct poly(pyrrole-3-carboxylic acid) (PP3C) film on a gold-coated grating substrate using pyrrole-3-carboxylic acid (P3C) monomer solution in 0.5 M H₂SO₄. In situ electrochemical-transmission surface plasmon resonance (EC-TSPR) measurements were carried out to study the kinetic and electroactivity properties of PP3C film. Immobilization of antihuman IgG on the activated surface and the binding process of human IgG and antihuman IgG in neutral solution could be detected in situ by EC-TSPR measurement. The surface modification steps on the PP3C layer led to an increase in intensity of the transmission peak. The performance, sensitivity, and utility of EC-TSPR spectroscopy showed obvious advantages for the detection of binding process with the simple experimental setup, and could be applied to the study of biomolecular interactions in various systems.

Keywords:

electrochemical-transmission surface plasmon resonance; grating; conduction polymer; poly(pyrrole-3-carboxylic acid); biosensor; human IgG