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Fast Determination of Adenosine 5鈥?Triphosphate (ATP) and Its Catabolites in Royal Jelly Using Ultraperformance Liquid Chromatography
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  • 作者:Ling Zhou ; XiaoFeng Xue ; JinHui Zhou ; Yi Li ; Jing Zhao ; LiMing Wu
  • 刊名:Journal of Agricultural and Food Chemistry
  • 出版年:2012
  • 出版时间:September 12, 2012
  • 年:2012
  • 卷:60
  • 期:36
  • 页码:8994-8999
  • 全文大小:212K
  • 年卷期:v.60,no.36(September 12, 2012)
  • ISSN:1520-5118
文摘
To obtain insight into the metabolic regulation of adenosine 5鈥?triphosphate (ATP) in royal jelly and to determine whether ATP and its catabolites can be used as objective parameters to evaluate the freshness and quality of royal jelly (RJ), a rapid ultraperformance liquid chromatography (UPLC) method has been developed for feasible separation and quantitation of ATP and its catabolites in RJ, namely, adenosine 5鈥?diphosphate (ADP), adenosine 5鈥?monophosphate (AMP), inosine monophosphate (IMP), inosine (HxR), and hypoxanthine (Hx). The analytes in the sample were extracted using 5% precooled perchloric acid. Chromatographic separation was performed on a Waters Acquity UPLC system with a Waters BEH Shield RP18 column and gradient elution based on a mixture of two solvents: solvent A, 50 mM phosphate buffer (pH 6.5); and solvent B, acetonitrile. The recoveries were in the range of 86.0鈥?02.3% with RSD of no more than 3.6%. The correlation coefficients of six analytes were high (r2 鈮?0.9988) and within the test ranges. The limits of detection and quantification for the investigated compounds were lower, at 0.36鈥?.68 and 1.22鈥?.30 mg/kg, respectively. The overall intra- and interday RSDs were no more than 1.8%. The developed method was successfully applied to the analysis of the analytes in samples. The results showed that ATP in RJ sequentially degrades to ADP, AMP, IMP, HxR, and Hx during storage.

Keywords:

ATP; catabolites; ultraperformance liquid chromatography; royal jelly

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