Black Tea Polyphenols Reverse Epithelial-to-Mesenchymal Transition and Suppress Cancer Invasion and Proteases in Human Oral Cancer Cells

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• 作者：Yu-Chao Chang ; Pei-Ni Chen ; Shu-Chen Chu ; Chin-Yin Lin ; Wu-Hsien Kuo ; Yih-Shou Hsieh
• 刊名：Journal of Agricultural and Food Chemistry
• 出版年：2012
• 出版时间：August 29, 2012
• 年：2012
• 卷：60
• 期：34
• 页码：8395-8403
• 全文大小：556K
• 年卷期：v.60,no.34(August 29, 2012)
• ISSN：1520-5118

文摘

Epithelial-to-mesenchymal transition (EMT) in cancer cells is considered to be a prerequisite for acquiring invasive/migratory phenotype and subsequent metastasis. This study provides molecular evidence associated with the antimetastatic effect of black tea polyphenol extracts (BTE), which contain polyphenols including gallic acid, gallocatechin, catechin, epigallocatechin-3-gallate, epicatechin-3-gallate, and theaflavin 3,3鈥?digallate, in an an oral squamous cell culture system by showing a nearly complete inhibition on the invasion (p < 0.001) of SCC-4 cells via reduced activities of MMP-2 (p < 0.001) and u-PA (p < 0.001). Immunoblot was performed to find that BTE could induce up-regulation of epithelial markers such as E-cadherin and inhibit mesenchymal markers such as snail-1 and vimentin. BTE inhibited p-FAK and p-paxillin, indicating the anti-EMT effect of BTE in oral squamous cell carcinoma. BTE was evidenced by its inhibition of the tumor growth of SCC-4 cells via cancer cell xenografted nude mice mode. These results suggested that BTE could reduce invasion by reversing EMT in human oral cancer cells.

Keywords:

EMT; invasion; MMP; u-PA; tea polyphenols