Discovery of SLC3A2 Cell Membrane Protein as a Potential Gastric Cancer Biomarker: Implications in Molecular Imaging

详细信息    查看全文

• 作者：Yixuan Yang ; Weiyi Toy ; Lee Yee Choong ; Peiling Hou ; Hassan Ashktorab ; Duane T Smoot ; Khay Guan Yeoh ; Yoon Pin Lim
• 刊名：Journal of Proteome Research
• 出版年：2012
• 出版时间：December 7, 2012
• 年：2012
• 卷：11
• 期：12
• 页码：5736-5747
• 全文大小：486K
• 年卷期：v.11,no.12(December 7, 2012)
• ISSN：1535-3907

文摘

Despite decreasing incidence and mortality, gastric cancer remains the second leading cause of cancer-related deaths in the world. Successful management of gastric cancer is hampered by lack of highly sensitive and specific biomarkers especially for early cancer detection. Cell surface proteins that are aberrantly expressed between normal and cancer cells are potentially useful for cancer imaging and therapy due to easy accessibility of these targets. Combining two-phase partition and isobaric tags for relative and absolute quantification methods, we compared the relative expression levels of membrane proteins between noncancer and gastric cancer cells. About 33% of the data set was found to be plasma membrane and associated proteins using this approach (compared to only 11% in whole cell analysis), several of which have never been previously implicated in gastric cancer. Upregulation of SLC3A2 in gastric cancer cells was validated by immunoblotting of a panel of 13 gastric cancer cell lines and immunohistochemistry on tissue microarrays comprising 85 matched pairs of normal and tumor tissues. Immunofluorescence and immunohistochemistry both confirmed the plasma membrane localization of SLC3A2 in gastric cancer cells. The data supported the notion that SLC3A2 is a potential biomarker that could be exploited for molecular imaging-based detection of gastric cancer.

Keywords:

iTRAQ; gastric cancer; plasma membrane; SLC3A2; biomarker