Viruslike Nanoparticles with Maghemite Cores Allow for Enhanced MRI Contrast Agents

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• 作者：Andrey G. Malyutin ; Rosemary Easterday ; Yaroslav Lozovyy ; Alessandro Spilotros ; Hu Cheng ; Olivia R. Sanchez-Felix ; Barry D. Stein ; David Gene Morgan ; Dmitri I. Svergun ; Bogdan Dragnea ; Lyudmila M. Bronstein
• 刊名：Chemistry of Materials
• 出版年：2015
• 出版时间：January 13, 2015
• 年：2015
• 卷：27
• 期：1
• 页码：327-335
• 全文大小：584K
• ISSN：1520-5002

文摘

Here, for the first time, we demonstrate formation of virus-like nanoparticles (VNPs) utilizing gold-coated iron oxide nanoparticles as cores and capsid protein of brome mosaic virus (BMV) or hepatitis B virus (HBV) as shells. Further, utilizing cryo-electron microscopy and single particle methods, we are able to show that the BMV coat on VNPs assembles into a structure very close to that of a native virion. This is a consequence of an optimal iron oxide NP size (鈭?1 nm) fitting the virus cavity and an ultrathin gold layer on the maghemite cores, which allows for utilization of SH-(CH₂)₁₁-(CH₂-CH₂-O)₄-OCH₂-COOH as capping molecules to provide sufficient stability, charge density, and small form factor. MRI studies show unique relaxivity ratios that diminish only slightly with gold coating. A virus protein coating of a magnetic core mimicking the wild-type virus makes these VNPs a versatile platform for biomedical applications.