Glycine-Terminated Dendritic Amphiphiles for Nonviral Gene Delivery

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• 作者：Shashwat Malhotra ; Hannah Bauer ; Ariane Tschiche ; Anna Maria Staedtler ; Andreas Mohr ; Marcelo Calder贸n ; Virinder S. Parmar ; Lena Hoeke ; Soroush Sharbati ; Ralf Einspanier ; Rainer Haag
• 刊名：Biomacromolecules
• 出版年：2012
• 出版时间：October 8, 2012
• 年：2012
• 卷：13
• 期：10
• 页码：3087-3098
• 全文大小：633K
• 年卷期：v.13,no.10(October 8, 2012)
• ISSN：1526-4602

文摘

Development of nonviral vectors for the successful application of gene therapy through siRNA/DNA transfection of cells is still a great challenge in current research.nclick="showRef(event, 'ref1 ref2'); return false;" href="JavaScript:void(0);" class="ref">(1, 2) In the present study, we have developed multivalent polyglycerol dendron based amphiphiles with well-defined molecular structures that express controlled glycine arrays on their surfaces. The structure鈥揳ctivity relationships with respect to the siRNA complexation, toxicity, and transfection profiles were studied with synthesized amphiphilic polycations. Our findings revealed that a second-generation amphiphilic dendrimer (G2-octaamine, 4) that has eight amine groups on its surface and a hydrophobic C-18 alkyl chain at the core of the dendron, acts as an efficient vector to deliver siRNA and achieve potent gene silencing by investigating the knockdown of luciferase and GAPDH gene activity in HeLa cells. Interestingly, the amphiphilic vector is nontoxic even at higher ratio of N/P 100. To the best of our knowledge this is the first example of successful in vitro siRNA transfection using dendritic amphiphiles. We believe that this supramolecular complex may serve as a new promising alternative for nonviral siRNA delivery systems and will be investigated for in vivo siRNA delivery in the future.