PEGylated Graphene Oxide-Mediated Protein Delivery for Cell Function Regulation

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• 作者：He Shen ; Min Liu ; Huixin He ; Liming Zhang ; Jie Huang ; Yu Chong ; Jianwu Dai ; Zhijun Zhang
• 刊名：ACS Applied Materials & Interfaces
• 出版年：2012
• 出版时间：November 28, 2012
• 年：2012
• 卷：4
• 期：11
• 页码：6317-6323
• 全文大小：421K
• 年卷期：v.4,no.11(November 28, 2012)
• ISSN：1944-8252

文摘

Delivery of proteins into cells may alter cellular functions as various proteins are involved in cellular signaling by activating or deactivating the corresponding pathways and, therefore, can be used in cancer therapy. In this study, we have demonstrated for the first time that PEGylated graphene oxide (GO) can be exploited as a nanovector for efficient delivery of proteins into cells. In this approach, GO was functionalized with amine-terminated 6-armed polyethylene glycol (PEG) molecules, thereby providing GO with proper physiological stability and biocompatibility. Proteins were then loaded onto PEG-grafted GO (GO-PEG) with high payload via noncovalent interactions. GO-PEG could deliver proteins to cytoplasm efficiently, protecting them from enzymatic hydrolysis. The protein delivered by GO-PEG reserves its biological activity that regulates the cell fate. As a result, delivery of ribonuclease A (RNase A) led to cell death and transport of protein kinase A (PKA) induced cell growth. Taken together, this work demonstrated the feasibility of PEGlyated GO as a promising protein delivery vector with high biocompatibility, high payload capacity and, more importantly, capabilities of protecting proteins from enzymatic hydrolysis and retaining their biological functions.

Keywords:

graphene oxide; loading capacity; protein delivery; cellular uptake; enzymatic hydrolysis; regulation of cell function