Dioxin-like Potency of HO- and MeO- Analogues of PBDEs鈥?the Potential Risk through Consumption of Fish from Eastern China

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• 作者：Guanyong Su ; Jie Xia ; Hongling Liu ; Michael H. W. Lam ; Hongxia Yu ; John P. Giesy ; Xiaowei Zhang
• 刊名：Environmental Science & Technology (ES&T)
• 出版年：2012
• 出版时间：October 2, 2012
• 年：2012
• 卷：46
• 期：19
• 页码：10781-10788
• 全文大小：338K
• 年卷期：v.46,no.19(October 2, 2012)
• ISSN：1520-5851

文摘

Polybrominated diphenyl ethers (PBDEs) and their analogues, such as hydroxylated PBDE (HO-PBDEs) and methoxylated PBDE (MeO-PBDEs) are of interest due to their wide distribution, bioaccumulation and potential toxicity to humans and wildlife. While information on the toxicity/biological potencies of PBDEs was available, information on analogues of PBDEs was limited. Dioxin-like toxicity of 34 PBDEs analogues was evaluated by use of the H4IIE-luc, rat hepatoma transactivation bioassay in 384-well plate format at concentrations ranging from 0 to 10鈥?00 ng/mL. Among the 34 target analogues of PBDEs studied here, 19 activated the aryl hydrocarbon receptor (AhR) and induced significant dioxin-like responses in H4IIE-luc cells. Efficacies of the analogues of PBDEs ranged from 5.0% to 101.8% of the maximum response caused by 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD-max) and their respective 2,3,7,8-TCDD potency factors (ReP_H4IIE-luc) ranged from 7.35 脳 10^鈥?2 to 4.00 脳 10^鈥?, some of which were equal to or more potent than some mono-ortho-substituted PCBs (TEF-_WHO = 3 脳 10^鈥?). HO-PBDEs exhibited greater dioxin-like activity than did the corresponding MeO-PBDEs. Analogues of PBDEs were detected mostly in marine organisms. Of these 11 detected analogues of PBDEs, 6 were found to have measurable dioxin-like potency. Though some analogues of PBDEs exhibited significant dioxin-like potency as measured by responses of the H4IIE-luc transactivation assay, concentrations of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) equivalents (^{PBDEs聽analogues}TEQ_H4IIE-luc), calculated as the sum of the product of concentrations of individual PBDE and their ReP_H4IIE-luc, were less than the tolerance limit proposed by European Union and the oral reference dose (RfD) derived by U.S. Environmental Protection Agency, respectively. (Hazard Quotients (HQ) < 0.005) Additional investigations should be conducted to evaluate the toxic potencies of these chemicals, especially for 2鈥?MeO-BDE-28, 4-HO-BDE-90, 6-HO-BDE-47, and 6-MeO-BDE-47, which had been detected in other environmental media, including human blood.