Quantifying Ligand Adsorption to Nanoparticles Using Tandem Differential Mobility Mass Analysis

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• 作者：Suvajyoti Guha ; Xiaofei Ma ; Michael J. Tarlov ; Michael R. Zachariah
• 刊名：Analytical Chemistry
• 出版年：2012
• 出版时间：August 7, 2012
• 年：2012
• 卷：84
• 期：15
• 页码：6308-6311
• 全文大小：213K
• 年卷期：v.84,no.15(August 7, 2012)
• ISSN：1520-6882

文摘

Although electrospray-differential mobility analyzers (ES-DMA) have been previously employed to characterize ligand binding to nanoparticles, absolute quantification of surface coverage can be inaccurate at times because of ligand conformational effects. In this Letter, we report a quantitative technique by in-flight coupling of a particle mass analyzer (APM) with ES-DMA, thus enabling a direct quantitative analysis of mass independent of particle size, material, morphology and conformation. We demonstrate the utility of ES-DMA鈥揂PM by studying two model complex systems (gold nanoparticle鈥揵ovine serum albumin and polystyrene bead鈥揳ntibody) as a function of concentration and pH. Our results obtained with ES-DMA鈥揂PM are in excellent agreement with prior work. We anticipate that this will enhance the capabilities of online quantitative characterization of ligand binding to nanoparticles.