Controlled-pH Tissue Cleanup Protocol for Signal Enhancement of Small Molecule Drugs Analyzed by MALDI-MS Imaging

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• 作者：Mohammadreza Shariatgorji ; Patrik K盲llback ; Lena Gustavsson ; Nicoletta Schintu ; Per Svenningsson ; Richard J.A. Goodwin ; Per E. Andren
• 刊名：Analytical Chemistry
• 出版年：2012
• 出版时间：May 15, 2012
• 年：2012
• 卷：84
• 期：10
• 页码：4603-4607
• 全文大小：287K
• 年卷期：v.84,no.10(May 15, 2012)
• ISSN：1520-6882

文摘

The limit of detection of low-molecular weight compounds in tissue sections, analyzed by matrix assisted laser desorption ionization mass spectrometry imaging (MALDI-MSI), was significantly improved by employing sample washing using a pH-controlled buffer solution. The pH of the washing solutions were set at values whereby the target analytes would have low solubility. Washing the tissue sections in the buffered solution resulted in removal of endogenous soluble ionization-suppressing compounds and salts, while the target compound remained in situ with minor or no delocalization during the buffered washing procedure. Two pharmaceutical compounds (cimetidine and imipramine) and one new protease inhibitor compound were successfully used to evaluate the feasibility of the pH-controlled tissue washing protocol for MALDI-MSI. Enhancement in signal-to-noise ratio was achieved by a factor of up to 10.