Polymeric Nanoparticles with Encapsulated Superparamagnetic Iron Oxide and Conjugated Cisplatin for Potential Bladder Cancer Therapy

详细信息    查看全文

• 作者：Chao Huang ; Koon Gee Neoh ; Liqun Xu ; En Tang Kang ; Edmund Chiong
• 刊名：Biomacromolecules
• 出版年：2012
• 出版时间：August 13, 2012
• 年：2012
• 卷：13
• 期：8
• 页码：2513-2520
• 全文大小：453K
• 年卷期：v.13,no.8(August 13, 2012)
• ISSN：1526-4602

文摘

Amphiphilic poly(蔚-caprolactone)-b-poly(propargyl methacrylate-click-mercaptosuccinic acid-co-poly(ethylene glycol) methyl ether methacrylate) (PCL-b-P(PMA-click-MSA-co-PEGMA)) were synthesized by a combination of ring-opening polymerization, reversible addition鈥揻ragmentation chain transfer (RAFT) polymerization, and thiol-yne 鈥渃lick鈥?reaction. The hydrophobic PCL core can be used to load superparamagnetic iron oxide nanoparticles (SPIONs), while the pendant dicarboxylic groups in the hydrophilic shell are used to coordinate cisplatin. These SPIONs-loaded, cisplatin-conjugated polymeric nanoparticles (Pt鈥揊e鈥揚Ns) are superparamagnetic at room temperature and are mucoadhesive. Release of cisplatin from Pt鈥揊e鈥揚Ns in artificial urine at 37 掳C was characterized by an initial burst release with a release of 30% of the cisplatin in the first 4 h followed by a slow sustained release over 4 days. The cisplatin release can be further enhanced by increasing the temperature. These Pt鈥揊e鈥揚Ns can effectively induce cytotoxicity against UMUC3 bladder cancer cells with IC₅₀ of 32.3 渭M. These results indicate that Pt鈥揊e鈥揚Ns is potentially a promising cisplatin delivery vehicle which can be combined with SPIONs-induced hyperthermia for bladder cancer therapy.