Pentafluorophenyl Ester-Functionalized Phosphorylcholine Polymers: Preparation of Linear, Two-Arm, and Grafted Polymer鈥揚rotein Conjugates

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• 作者：Samantha McRae ; Xiangji Chen ; Katrina Kratz ; Debasis Samanta ; Elizabeth Henchey ; Sallie Schneider ; Todd Emrick
• 刊名：Biomacromolecules
• 出版年：2012
• 出版时间：July 9, 2012
• 年：2012
• 卷：13
• 期：7
• 页码：2099-2109
• 全文大小：585K
• 年卷期：v.13,no.7(July 9, 2012)
• ISSN：1526-4602

文摘

Novel pentafluorophenyl (PFP)-ester-functionalized phosphorylcholine (PC) polymers of different architectures were prepared and conjugated to lysozyme as a model protein. Linear and two-arm poly(2-methacryloyloxyethyl phosphorylcholine) (polyMPC) structures containing PFP functionality at the chain-end were prepared by atom transfer radical polymerization (ATRP) from novel initiators. Additional conjugates were prepared from phosphorylcholine-substituted cyclooctene (PC-COE) polymers containing PFP-ester bearing comonomers. The polymer鈥損rotein conjugates were characterized by HPLC, FPLC, and DLS and were seen to retain most (80% or greater) of their native enzymatic activity. Pharmacokinetic profiles of the polymer鈥損rotein conjugates were studied in mice and found to increase the circulation half-life compared with lysozyme alone.