Rapid Estimation of Glucosinolate Thermal Degradation Rate Constants in Leaves of Chinese Kale and Broccoli (Brassica oleracea) in Two Seasons

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• 作者：Kristin Hennig ; Ruud Verkerk ; Guusje Bonnema ; Matthijs Dekker
• 刊名：Journal of Agricultural and Food Chemistry
• 出版年：2012
• 出版时间：August 15, 2012
• 年：2012
• 卷：60
• 期：32
• 页码：7859-7865
• 全文大小：362K
• 年卷期：v.60,no.32(August 15, 2012)
• ISSN：1520-5118

文摘

Kinetic modeling was used as a tool to quantitatively estimate glucosinolate thermal degradation rate constants. Literature shows that thermal degradation rates differ in different vegetables. Well-characterized plant material, leaves of broccoli and Chinese kale plants grown in two seasons, was used in the study. It was shown that a first-order reaction is appropriate to model glucosinolate degradation independent from the season. No difference in degradation rate constants of structurally identical glucosinolates was found between broccoli and Chinese kale leaves when grown in the same season. However, glucosinolate degradation rate constants were highly affected by the season (20鈥?0% increase in spring compared to autumn). These results suggest that differences in glucosinolate degradation rate constants can be due to variation in environmental as well as genetic factors. Furthermore, a methodology to estimate rate constants rapidly is provided to enable the analysis of high sample numbers for future studies.

Keywords:

Brassicaceae; glucosinolates; glucosinolate breakdown; kinetic modeling; model discrimination; Brassica oleracea var. alboglabra; Brassica oleracea var. italica; seasonal effects; thermal degradation