Comparative Proteomic Analysis of Mushroom Cell Wall Proteins among the Different Developmental Stages of Pleurotus tuber-regium

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• 作者：Lei Chen ; Bo-Bo Zhang ; Peter C. K. Cheung
• 刊名：Journal of Agricultural and Food Chemistry
• 出版年：2012
• 出版时间：June 20, 2012
• 年：2012
• 卷：60
• 期：24
• 页码：6173-6182
• 全文大小：265K
• 年卷期：v.60,no.24(June 20, 2012)
• ISSN：1520-5118

文摘

Cell wall proteins (CWPs) play a vital role in the development of the different morphological stages including mycelium, fruiting body, and sclerotium in mushrooms which are important human food sources. Using fractionation by detergents and reducing agents, mushroom cell wall fractions from the different developmental stages of Pleurotus tuber-regium (PTR) were prepared. Using one-dimensional gel electrophoresis coupled with LC鈥揗S, there were 103, 91, and 48 noncovalently linked CWPs identified in the cell wall fractions of the PTR mycelium, fruiting body, and sclerotium, respectively. Comparing the CWPs in these cell wall fractions, 19 of them were in common, among which 17 belonged to the functional categories of translation, ribosomal structure, and biogenesis. This is the first study to provide important biochemical insights into the different developmental stages of PTR mediated by CWPs, and the identified CWPs helped to explain the morphological changes of PTR mushrooms during cultivation.

Keywords:

mushroom; cell wall proteins; proteomic analysis; Pleurotus tuber-regium