Feasibility Study on the Use of Visible鈥揘ear-Infrared Spectroscopy for the Screening of Individual and Total Glucosinolate Contents in Broccoli

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• 作者：Jos茅 Miguel Hern谩ndez-Hierro ; Juan Valverde ; Salvador Villacreces ; Kim Reilly ; Michael Gaffney ; Maria Lourdes Gonz谩lez-Miret ; Francisco Jos茅 Heredia ; Gerard Downey
• 刊名：Journal of Agricultural and Food Chemistry
• 出版年：2012
• 出版时间：August 1, 2012
• 年：2012
• 卷：60
• 期：30
• 页码：7352-7358
• 全文大小：327K
• 年卷期：v.60,no.30(August 1, 2012)
• ISSN：1520-5118

文摘

The potential of visible鈥搉ear-infrared spectroscopy to determine selected individual and total glucosinolates in broccoli has been evaluated. Modified partial least-squares regression was used to develop quantitative models to predict glucosinolate contents. Both the whole spectrum and different spectral regions were separately evaluated to develop the quantitative models; in all cases the best results were obtained using the near-infrared zone between 2000 and 2498 nm. These models have been externally validated for the screening of glucoraphanin, glucobrassicin, 4-methoxyglucobrassicin, neoglucobrassicin, and total glucosinolates contents. In addition, discriminant partial least-squares was used to distinguish between two possible broccoli cultivars and showed a high degree of accuracy. In the case of the qualitative analysis, best results were obtained using the whole spectrum (i.e., 400鈥?498 nm) with a correct classification rate of 100% in external validation being obtained.

Keywords:

glucosinolates; broccoli; visible spectroscopy; near-infrared spectroscopy; chemometrics