Improved Purity and Immunostimulatory Activity of 尾-(1鈫?)(1鈫?)-Glucan from Pleurotus sajor-caju Using Cell Wall-Degrading Enzymes

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• 作者：Saranya Satitmanwiwat ; Khanok Ratanakhanokchai ; Natta Laohakunjit ; Louis Kuoping Chao ; Shui-Tein Chen ; Patthra Pason ; Chakrit Tachaapaikoon ; Khin Lay Kyu
• 刊名：Journal of Agricultural and Food Chemistry
• 出版年：2012
• 出版时间：May 30, 2012
• 年：2012
• 卷：60
• 期：21
• 页码：5423-5430
• 全文大小：358K
• 年卷期：v.60,no.21(May 30, 2012)
• ISSN：1520-5118

文摘

The objective of this work was to improve the purity of 尾-(1鈫?)(1鈫?)-glucan in the native triple helical structure from the fruiting bodies of Pleurotus sajor-caju for effective biological function using cell wall-degrading enzymes. A crude carbohydrate was extracted with hot water, then treated with crude xylanase and cellulase from Paenibacillus curdlanolyticus B-6. 尾-Glucan in the extract was purified to homogeneity with a single and symmetrical peak using 650M DEAE Toyopearl and Sepharose CL-6B column chromatography. The purity of 尾-glucan was confirmed by high-performance size-exclusion chromatography. Purified 尾-glucan was obtained at a purity of up to 90.2%. The Congo red reaction and atomic force microscopy indicated that the purified 尾-glucan exhibited a triple helix conformation. Purified 尾-glucan was able to effectively up-regulate the functions of macrophages such as nitric oxide (NO) and tumor necrosis factor (TNF-伪) production.

Keywords:

cellulase; cell wall-degrading enzymes; immunostimulatory activity; Pleurotus sajor-caju; xylanase; 尾-(1鈫?)(1鈫?)-glucan