Interchenar Retrotransfer of Aureothin Intermediates in an Iterative Polyketide Synthase Module

详细信息    查看全文

• 作者：Benjamin Busch ; Nico Ueberschaar ; Yuki Sugimoto ; Christian Hertweck
• 刊名：The Journal of the American Chemical Society
• 出版年：2012
• 出版时间：August 1, 2012
• 年：2012
• 卷：134
• 期：30
• 页码：12382-12385
• 全文大小：292K
• 年卷期：v.134,no.30(August 1, 2012)
• ISSN：1520-5126

文摘

The course of the enigmatic iterative use of a polyketide synthase module was deduced from targeted domain inactivation in the aureothin assembly line. Mutational analyses revealed that the N-terminus of AurA is not involved in the iteration process, ruling out an ACP鈥揂CP shuttle. Furthermore, an AurA(KS掳, ACP掳)鈥揂urA(AT⁰) heterodimer proved to be nonfunctional, whereas aureothin production was restored in a 螖aurA mutant complemented with AurA(KS掳)鈥揂urA(ACP掳). This finding supports a model according to which the ACP-bound polyketide intermediate is transferred back to the KS domain on the opposite PKS strand.